Induction of pluripotent stem cells from adult human fibroblasts

Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. In the s, some other transcription factors were discovered, particularly globin transcription factor 1 Gata-1 , that can reprogram avian monocyte precursors into erythrocytes, eosinophils, and megakaryocytes Kulessa et al. We named the cells "induced pluripotent stem cells" iPS cells. The first attempt of the reprogramming technique was performed by Robert Briggs and Thomas King. Autogenous bone grafts are the gold standard for such reconstruction because of their osteoconductive, osteoinductive and nonimmunogenic properties. Generation of human-induced pluripotent stem cells. Find all citations in this journal default.
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Subscribe to our newsletter to receive the latest articles and DTScience opportunities. Molecular therapy : the journal of the American Society of Gene Therapy. The ability of iPS cells to be cultured in vitro in a pluripotent state and the potential to differentiate them into any cell type has made them a highly sought after research tool. User comments must be in English, comprehensible and relevant to the article under discussion. Nat Cell Biol ;13 5
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Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. The process of reprogramming a somatic cell into an iPS cell and back to a differentiated cell requires relatively few manipulations. When the cells reached subconfluence, they were dissociated using 0. After several passages, ESC-like colonies were selected for further cultivation and characterization. An example of the medical application of iPS cell technology was demonstrated by Itzhaki et al. Biochemical and biophysical research communications.
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What are the hoped-for or already realized applications for these pluripotent stem cells? The newly inserted gene endowed mice with resistance to an antibiotic named G These findings demonstrate that iPS cells can be generated from adult human fibroblasts. Progress in understanding reprogramming to the induced pluripotent state. A comment on this article appears in " Achieving pluripotency. Support Contact Us Corporate website. Compared with other transfection vectors, Sendai virus exhibits a high transfection efficiency and is not pathogenic to humans
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